PhotoMax 200
PHOTO DIODE DETECTION SYSTEM
A high speed (high sample rate), low noise, dedicated Photometry (Photo Diode) system using fluorescence measurements for acquiring fast time-series images from biological preparation
Applications:
· Detection (confocal) of localized Ca transients
· Cell detection of Ca transients (global); pH changes;
changes in light scattering, birefringence, dichroism, etc.
· Fluorescence changes related to site-directed probes:
FRET and LRET
· Absorbance or fluorescence signals reported by
potentiometric dyes
· Fluorescence intensity changes reported by few
molecules, or single molecule (photon counting)
· Glitch free detection of optical signals associated
with flash-induced Photo-release of bioactive molecules (flash
photolysis)
Features:
· Track and hold to subtract background current
· Up to 300 volt diode bias voltage (for avalanche
diode)
· Ultra low noise current to voltage converter (pure
integrating and mixed integrating)
· RMS noise meter
· Local and remote gain control
· Four pole Bessel filter
· External controllable resets for integrating mode.
Allows for input blanking of flash artifact
Two Headstage Types:
· PIN diode current amplifier. Comes standard configured for global light detection (adjustable focusing lens directs all available light in viewing area to photo diode). Also Available 30 or 50 micron pin holed PIN Photo diodes for spot detection
· APD, Avalanche Photo Diode (two stage Peltier cooled)
Each Headstage comes complete with photo diode and Low noise integrating current to voltage converter.
Advantages:
· Low Noise, High sensitivity
· Dedicated Instrument with Photo Diode detector, and
Amplifier together in one Headstage
· Low Noise Integrating Current to Voltage Converter
· Cost Effective
D.A. Digregorio, A. Peskoff, J.L. Vergara (1999) Measurement
of action potential-induced presynapic calcium domains at a cultured
neuromuscular junction. J neurosci 19(18):7846-7859
A.M. Kim, J.L. Vergara (1998) Supercharging accelerates
T-tuule membrane potential changes in voltage clamped frog skeletal
muscle fibers. Biophys J 75:2098-2116
Cha, A. and F. Bezanilla, "Structural implications of
fluorescence
quenching in the Shaker K+ channel". J. Gen Physiol. 112:
391-408. (1998)
A.L. Escobar, P. Velez, A.M. Kim, F. Cafuentes, M.Fill, J.L.
Vergara (1997) Kenectic properties of DM-nitrophen and calcium
indicators: rapid transient response to plash photolysis. Eur J Physiol
434:615-631
Introductory Pricing:
| PhotoMax-200/PIN | PhotoMax 200 Integrating Photo Diode amplifier with PIN diode Headstage. Headstage is configured for global detection with PIN-08-GL and focusing lens. Includes three-axis translator and C-mount adapter |
$9,850.00
|
| PhotoMax-200/APD | PhotoMax 200 Integrating Photo Diode amplifier with two stage Peltier cooled Avalanche Photo Diode headstage. Includes three-axis translator and C-mount adapter |
$11,450.00
|
| PhM-PIN | Integrating Headstage with PIN diode. (includes PIN-08-GL and focusing lens) |
$1,995.00
|
| PhM-APD | Integrating Headstage with two stage Peltier cooled Avalanche Photo Diode. |
$3,595.00
|
| PIN-08-GL | Mounted HR-008 photo diode configured for global detection |
$195.00
|
| PIN-08-30 | Mounted HR-008 photo diode with 30 micron aperture pin hole for spot detection |
$395.00
|
| PIN-08-50 | Mounted HR-008 photo diode with 50 micron aperture pin hole for spot detection |
$395.00
|