CA-1B High Performance Oocyte Clamp
The CA-1B is the Worlds quietest and fastest Oocyte Clamp,
and it does both Cut Open Vaseline Gap and classical Two Electrode
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Press HERE for full size front panel (46K file size) |
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Features ...
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Modes of Operation: Conventional Two Electrode Clamp with 145 volt compliance and
three time constant Cm compensation. These capabilities are combined
with a very high performance bath clamp that enables you to clamp twice
as fast as any two electrode clamp available to date. A new unique Cut-open Oocyte Vaseline-gap method that is both very quiet, and is also fifty times faster (20 to 100 µs to change the membrane capacitance) than any oocyte clamp ever built. With this mode of operation, it is now also possible to control the intracellular milieu by internal perfusion of the oocyte. Now, stable recording lasting for several hours is possible. These properties allow the resolution of the time course of fast ionic and gating charge currents. Current noise is only about 1nA RMS at 3kHz bandwidth: a significant improvement over conventional two electrode oocyte clamps. CUT-OPEN Mode (Headstage) In this mode the Mainframe Clamp Amplifier is replaced by the Clamp Amplifier in the V1 Headstage. This eliminates much of the feedback path from V1 electrode (Voltage Monitoring) to I(Current Injecting) output. It improves speed and makes the CUT-OPEN operation even lower in noise (by a factor of 2 or 3 times!!) |
The CA-1B is designed to be easy to use, yet provide excellent performance for the most demanding applications. The CA-1 was designed by Dr. Enrico Stefani in collaboration with Dr. Francisco Bezanilla, both with the UCLA School of Medicine. To view experimental data obtained by them using a CA-1, click here. In addition to the TEV mode, there have been many enhancements to Series Resistance compensation since publication of this technique. And now there is also a Processed Output Section. |
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Click here to view more illustrations |
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Temperature Control The Cut-Open Chambers can also be temperature-controlled by using any of the three Dagan Temperature Controllers (TC-10, HCC-100A, or the DTC-200) along with the specially designed HE-104C Thermal Stage.
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Cut Open Chamber Configurations and Accessories
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Standard Cut-Open Chamber and Accessories Packages |
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CC Package A |
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This package includes a standard perfusion chamber and accessories for non-temperature controlled applications |
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CCP-2D |
Cut Open Perfusion Chamber |
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PL-6 |
Ag/AgCl Pellet Assembly |
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MS-104 |
Mounting Stage |
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PS-104 |
Plastic Stand |
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CC Package B |
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This package includes a thermally-conductive perfusion chamber, thermal stage and accessories for temperature controlled applications (Note: DOES NOT include a temperature controller.) |
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CCP-2D-T |
Thermally-conductive Perfusion Chamber |
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PL-6 |
Ag/AgCl Pellet Assembly |
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HE-104C |
Thermal Stage (mates to any of the Dagan Temperature Controllers) |
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PS-104 |
Plastic Stand |
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APPLICATION EXAMPLE To view an application example showing the improved results obtained when a temperature controller is used in conjunction with a voltage clamp experiment, click here. |
REFERENCES For a list of references using the CA-1B as well as a link to excellent techniques publications, click here. |
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CA-1B/CS |
'Clampator One' High Performance Oocyte Clamp (115 or 230 V AC) with all headstages (no chamber) |
$10,980.00 |
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CA-1B/NT |
Same as above, but without TEV mode |
$10,380.00 |
| CA-1B/HS | Complete Set of Headstages Only (Vi, V1/i, V2, BathGuard I/V) |
$2,540.00
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CC-1D |
Plastic Chamber Assembly for Cut-Open mode of operation, with pre-drilled holes |
$ 640.00 |
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CCP-2D |
Plastic Chamber Assembly (same as above), with perfusion apparatus |
$ 1,270.00 |
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CC-1 |
Plastic Chamber Assembly Only, without pre-drilled holes |
$ 400.00 |
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T Option |
Above Chambers also available with a thermal-conductive main chamber; add "-T" to the end of above part number (ie., CCP-2D-T), and add.. |
$ 200.00 |
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PL-6 |
6 Ag/AgCl pellet assemblies in block with 6 wells to couple to the above cut-open chambers |
$ 290.00 |
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PL-1 |
Replacement Ag/AgCl pellet for PL-6 |
$ 30.00 |
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MS-104 |
Chamber Mounting Stage for non-temperature controlled applications |
$ 95.00 |
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HE-104C |
Chamber Thermal Stage for temperature controlled applications |
$ 890.00 |
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PS-104 |
Plastic Stand for the MS-104 or the HE-104C |
$ 145.00 |
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CC Pkg. A |
Perfusion Chamber and Accessories for non-temperature controlled applications |
$1,540.00 |
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CC Pkg. B |
Thermally-conductive Chamber and Accessories for temperature controlled applications |
$2,330.00 |
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Other Two Electrode Clamps:
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Warning: This equipment is not intended for use in human applications or human experimentation.
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Dagan Corporation Minneapolis MN USA 612-827-5959 (FAX 612-827-6535) Email: info@dagan.com 7/08